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Xenomitochondrial mice, harboring evolutionarily divergent Mus terricolor mitochondrial DNA (mtDNA) on a Mus musculus domesticus nuclear background (B6NTac(129S6)-mt M. terricolor /Capt; line D7), were subjected to molecular and phenotypic analyses. No overt in vivo phenotype was identified in contrast to in vitro xenomitochondrial cybrid studies. Microarray analyses revealed differentially...
A novel flow-through device for performing fast PCR thermal cycling is presented. The thermal gradient thermal cycling device is comprised of layers of highly thermally conducting material separated by insulating layers. Channels etched in the conducting and insulating layers create one continuous path through the device. When the device is held between platens at different temperatures and PCR sample...
We detect single nucleotide polymorphisms (SNP) in genomic DNA that has been amplified using a standard PCR protocol where a short fluorescently tagged probe sequence and a single denaturation and annealing step have been added. We utilize the ability of ionically treated gold nanoparticles to selectively adsorb unhybridized probes in the modified PCR product and quench their fluorescence. The method...
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