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Overexpression of Bcl-xl, a member of the Bcl-2 protein family, is reported to protect from a variety of stresses involving delayed cell death. We tested the ability of Bcl-xl overexpression to protect primary cultures of embryonic rat septal neurons subjected to one of four different stresses: 6 h of combined oxygen–glucose deprivation, which produces rapid cell death, or a 24 h exposure to hypoglycemia,...
The mechanisms underlying resealing of transected myelinated rat dorsal root axons were investigated in vivo using an assay based on exclusion of a hydrophilic dye (Lucifer Yellow-biocytin conjugate). Smaller caliber axons (<5μm outer diameter) resealed faster than larger axons. Resealing was Ca 2+ dependent, requiring micromolar levels of extracellular [Ca 2+ ] to proceed,...
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