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A comparison of amino acid sequences of fungal chitosanases, belonging to family 75 of glycosyl hydrolases, revealed three carboxylic amino acid residues completely conserved among all of the chitosanases. To study the role of these residues in catalysis, they were replaced with other residues by site-directed mutagenesis in the chitosanase gene of Fusarium solani. The mutated genes were expressed...