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A simple, precise, accurate, and stability-indicating method is developed and validated for analysis of tetrahydrozoline hydrochloride in eye drop formulations. Separation was achieved on a reversed-phase C8 column (125mm×4.6mm i.d., 5μm) using a mobile phase consisting of acetonitrile/phosphate buffer of pH 3.0 (20:80, v/v) at a flow rate of 1.0mL/min and UV detection at 240nm. This method is validated...