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A new type II restriction endonuclease Asp7/8% has been isolated from the non-pathogenic species of Achromobacter 718. This novel enzyme, an isoschizomer of Kpnl, recognizes and cleaves specifically within the nucleotide sequence: 5'-G/GTAC-C-3' 3'-C-CATG/G-5'.In contrast to Kpnl, Asp718 generates fragments with 5'-protruding single-stranded ends. These 5'-terminal extensions of the Asp718 fragments...