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Initiation of motility in salmonid sperm is sensitive to the pH of the extracellular medium, however, the basis of this sensitivity is not clear. Sperm incubated in an immobilization buffer (SI) at low pH (∼7.1–7.2) become motile when diluted with activating medium (AM) at high (∼8.5) but not low pH. Based on this observation, various agents were tested to determine whether the onset of steelhead...