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Enterovirus 71 (EV71) infections in children manifest as exanthema and are most commonly known as hand-foot-and-mouth disease (HFMD). Because it can cause severe neurological complications like poliomyelitis, EV71 has now emerged as an important neurotropic virus in Asia. EV71 virus has been shown to consist of 3 (A, B and C) genotypes and many subgenotypes. Although EV71 vaccine development has recently...
The bacteriophage T7 expression system is one of the most popular methods used to produce recombinant proteins in prokaryotic cells. IPTG is generally used as the inducer. Without the reconstruction of expression vector or the use of different expression system, a novel induction strategy to enhance the level of protein expression is reported in this study. Our results show that the yield of purified...
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