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Specific polyclonal antibodies against the herbicide butachlor [2-chloro-N-(2,6-diethylphenyl)-N-(butoxymethyl)acetanilide] were obtained from rabbits immunized with butachlor-3-mercaptopropionic acid (BMPA), coupled to bovine serum albumin (BSA) and soybean trypsin inhibitor. Competitive enzyme-linked immunosorbent assays with photometric detection based on either immobilized antibody (direct assay)...
Two fluoroimmunoassays for detection of 2,4-dichlorophenoxyacetic acid (2,4-D) in an apolar organic solvent, reverse micelles of Aerosol OT in n-octane, are proposed. Both assays are homogeneous methods, meaning that no separation of free and bound fraction of analyte and no washing steps are required. To perform the assay just add the fluorescein-labeled 2,4-D (FD) and monoclonal antibody to the...
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