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A recent controversial report suggests that the nocturnal inhibitor of Rubisco, 2-carboxy-d-arabinitol 1-phosphate (CA1P), does not bind to Rubisco in vivo and therefore that CA1P has no physiological relevance to photosynthetic regulation. It is now proved that a direct rapid assay can be used to distinguish between Rubisco-bound and free CA1P, as postulated in the controversial report. Application...