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A highly stable and sensitive amperometric ethanol bi-enzyme biosensor was developed using alcohol oxidase isolated from thermotolerant methylotrophic yeast Hansenula polymorpha and horseradish peroxidase as biorecognition elements. Enzyme immobilization was performed by means of electrodeposition paints (EDP) with a first layer integrating horseradish peroxidase within an Os-complex modified EDP...