The Infona portal uses cookies, i.e. strings of text saved by a browser on the user's device. The portal can access those files and use them to remember the user's data, such as their chosen settings (screen view, interface language, etc.), or their login data. By using the Infona portal the user accepts automatic saving and using this information for portal operation purposes. More information on the subject can be found in the Privacy Policy and Terms of Service. By closing this window the user confirms that they have read the information on cookie usage, and they accept the privacy policy and the way cookies are used by the portal. You can change the cookie settings in your browser.
Protein structure comparison algorithms are useful for predicting aspects of protein function. Some algorithms identify remote homologs, while others distinguish closely related proteins that prefer different substrates. Most of these methods assume that proteins are rigid in order to perform comparisons more rapidly, while others compensate for flexibility by representing proteins as a connected...
In this study, we proposed an approach for constructing directed, regulatory gene set networks to reveal novel relationships among gene sets. Results from this study showed that regulatory gene set networks can provide complementary information to existing types of gene set networks and explain underlying mechanisms of a disease.
Liquid chromatography-based tandem mass spectrometry (LC-MS) technique allows for identification and quantification of thousands of proteins in parallel. This technique coupled with a feed-forward artificial neural network provides a technique to analyze and select protein panels for use in multi-biomarker panel discovery applications. In this study, we enhance this technique by utilizing massively...
This study is based on a simple hypothesis - “ideal” drugs for a patient can cure the patient's disease by modulating the gene expression profile of the patient to a similar level with those in healthy people, on the pathway level. To verify this hypothesis, we present a computational framework to evaluate drug effects on gene expression profiles in breast cancer. First, a breast cancer pathway model...
Regarding the issue that 3D protein structure similarity determination requires a lot of computing time, this paper proposes a 3D protein structure consistency comparison using 2nd PCA (second principal component analysis). Mainly through 3D protein structure distance matrix to compute the corresponding PCA2 value, define their similarity by the PCA2 ratio of the two protein structures to be compared...
MAPK cell signal transduction pathways determine the survival of the cells. If one can control this pathways, and then they will prohibit the proliferation of the cancer cells. Furthermore, they will heal the cancer smoothly. In order to attain this goal, we use lots of drugs to interact with MEK1 in MAPK, by using computer aided drug design to analyze the ligand activity of proteins in MEK1.
In this work we present preliminary results on the optical response and sensitivity tests of waveguide-based photonic crystal structures acting as biosensors for proteins and oligonucleotides. Several one-and two-dimensional Silicon-on-insulator (SOI) PhCs with lattice constants in the submicron range have been realized by means of electron-beam lithography and reactive ion etching techniques.
We report on a new method to handle fluids in microfluidic systems by using both micro- aspiration and PDMS valves. Multilayer soft lithography technique has been used to fabricate microfluidic circuit and micro-aspiration network. We introduced micro-aspiration areas to locally create vacuum in the microfluidic network. The induced pressure differences can drive confined liquid into motion. This...
This research is to develop a system to allow cancer researchers as well as drug discovery people to study drug effects in situ, real-time and at "few cells" level but large cell numbers. A microelectrode array was designed and fabricated by using silicon micromachining technology. The cancer cell NCI-H460 was attached on the Au microelectrode and the high impedance sensitivity at very "few...
The investigation of interactions between two kinds of monoclonal antibodies and SARS virus with a label-free protein array technique were presented in this paper. The performance consists of three parts: a surface modification for ligand immobilization/surface, a protein array fabrication with an integrated microfluidic system for patterning, packaging and liquid handling, and a protein array reader...
The concept of biosensor with imaging ellipsometry was proposed about ten years ago. It has become an automatic analysis technique for protein detection with merits of label-free, multi-protein analysis, and real-time analysis for protein interaction process, etc. Its principle, and related technique units, such as micro-array, micro-fluidic and bio-molecule interaction cell, sampling unit and calibration...
Set the date range to filter the displayed results. You can set a starting date, ending date or both. You can enter the dates manually or choose them from the calendar.