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The binding of nucleotides to pig heart nucleoside-diphosphate kinase was studied using Rose Bengal as an optical probe. ATP, in the absence of Mg 2+ , binds slowly to the enzyme, with a second order rate constant of about 3000 M -1 .s -1 , whereas in its presence the binding is much faster. This finding suggests the regulation of the nucleoside-diphosphate kinase...