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At thermoneutral conditions, high steady-state levels of transcripts for both IL-1α and its receptor IL-1RtI were found in specialized thermogenic organ, brown adipose tissue (BAT) of adult mice, as compared with the levels in lymph nodes, brain and spleen. A pronounced decrease of IL-1α mRNA level in BAT was observed after lipopolysaccharide (LPS) administration and after exposure to cold. Likewise,...
The half-life of the F 1 -ATPase β-subunit (F 1 -β) mRNA in ATPase-poor brown adipose tissue (BAT) (t?? = 9.5 h) was found to be 3-7-fold shorter than in liver (t?? = 27 h) and heart (t?? = 63 h) of mice. When translated in reticulocyte lysate, a 2-3-fold lower efficiency appeared with F 1 -β mRNA from BAT than from other tissues. The in vitro synthesized F 1 -β protein...
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