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This paper reports the four-part differential leukocyte count (DLC) of human blood using a MEMS microflow (μflow) cytometer. It is achieved with a two-color laser-induced fluorescence (LIF) detection scheme. Four types of leukocytes including neutrophils, eosinophils, lymphocytes and monocytes are identified in blood samples, which are stained by fluorescein isothiocyanate (FITC) and propidium iodide...
In this paper, we demonstrated leukocyte labeling, sensing and differentiation from undiluted human whole blood samples with microfabricated devices. A challenging issue in leukocyte sensing from blood samples is the required high-dilution level, which is used mainly to prevent interference from the overwhelmingly outnumbered erythrocytes. Dilution is undesirable for micro hemacytometers. It not only...
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