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Yessotoxin (YTX) and its analogues are a newly recognized group of toxins with increased presence in shellfish in recent years. They can be quantified by various functional assays due to their interaction with phosphodiesterases (PDEs). One of these assays detects the binding between the YTX and the fluorescently labeled PDE I using fluorescence polarization, a spectroscopic technique based on exciting...
Marine algal toxins are an important cause of seafood-associated outbreaks. Some marine bacteria living in association with algae are able to produce channel-blocking substances similar to PSP and TTX toxins and a role of these bacteria in the toxicity of dinoflagellates has been hypothesized. The aim of this study was to monitor, over a period of 2 years, areas used in shellfish production in the...
Histological and immunocytochemical investigations were performed on different organs (brain, duodenum and thymus) of mice following lethal (420 μg/kg) or sublethal (10 μg/kg) intraperitoneal injection of yessotoxin (YTX). No morpho-functional modifications were observed in large neurons of the cerebral and cerebellar cortex with the sub-lethal dose, nor in the cerebral cortex with the lethal dose...
Swiss CD1 mice died less than 2 h after intraperitoneal injection of 420 μg/kg of algal yessotoxin (YTX). The morphological, histochemical and immunocytochemical studies performed on the cerebellar cortex revealed damage to the Purkinje cells. The main cytological alterations were observed in the cytoplasm, while less sufferance was detected in the nucleus. The immunocytochemical experiments showed...
This study reports, for the first time, immunocytochemical evidence of the distribution of algal yessotoxins (YTXs) in the mussel Mytilus galloprovincialis. Immunopositivity to YTXs was found in immunocytes and in the digestive gland. With regards the gland, the positivity was mainly present in the lumen of both tubules and ducts. No YTXs were detected in the gonads, while the presence of toxins in...
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