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p-Nitrobenzyl esters serve as protecting groups on intermediates in the manufacture of clinically important oral β-lactam antibiotics; de-esterification of the intermediates is required for synthesis of the final product. A Bacillus subtilis PNB carboxy-esterase (PNBCE) catalyzes hydrolysis of several β-lactam antibiotic PNB esters to the corresponding free acid and PNB alcohol. This communication...