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Pretreatment with 1nM 1,25-dihydroxyvitamin D 3 (1,25), or non-hypercalcemic Vitamin D analogs, upregulated the response of creatine kinase (CK) to 17β-estradiol (30nM E 2 ), raloxifene (3000nM RAL) or dihydrotestosterone (300nM DHT) in primary human bone cells. Previously, we reported that these osteoblast-like cells responded to gonadal steroids in a sex specific manner. Bone cells...
We have reported that pretreatment with 1α, 25(OH) 2 D 3 (1,25) up-regulates responsiveness and sensitivity to 17β estradiol (E 2 ) in osteoblast-like cells, as measured by parallel stimulation of [ 3 H]thymidine incorporation into DNA and the specific activity of creatine kinase BB (CK). Increased responsiveness was correlated with increased E 2 receptor concentration...
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