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Phages of low virulence predominated culturable phage populations in a naturally occurring Jamaican salt pond with Halobacterium cutirubrum as host. These mutated rapidly in culture to higher virulence due to more rapid adsorption to host cells. Wild-type phages of low virulence, S50.2 and S41, with adsorption rate constants (K) of 1.15 and 1.21 10 -11 ml min -1 mutated...
We describe several new cloning vectors for mutagenesis and allele replacement experiments. These plasmids have the R6Kγ DNA replication origin (oriR R6Kγ ) so they replicate only in bacteria supplying the Π replication protein (encoded bypir), and they can be maintained at low or high plasmid copy number by usingEscherichia colistrains encoding either wild-type or mutant forms of Π. They...
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