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An expression plasmid for mammalian cells (CLDN10B) has been modified to add nucleotides encoding hexahistidine and the FLAG peptide (H/F) to cDNAs. The new mammalian expression plasmid has been named pDouble Trouble (pDT). The plasmid and a recombinant baculovirus were used to produce native- and H/F-human A 1 and A 2A adenosine receptors, optimally expressed in CHO-K1 and Sf9...
A bovine brain adenosine A 1 receptor cDNA encoding a 326 amino acid protein has been identified. This cDNA, which encodes a protein >90% identical to analogous rat and dog receptors, was transiently expressed in COS-1 cells. Recombinant receptors exhibited the features of bovine A 1 receptors that distinguish it from rat and canine receptors, including subnanomolar K i ...
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