The Infona portal uses cookies, i.e. strings of text saved by a browser on the user's device. The portal can access those files and use them to remember the user's data, such as their chosen settings (screen view, interface language, etc.), or their login data. By using the Infona portal the user accepts automatic saving and using this information for portal operation purposes. More information on the subject can be found in the Privacy Policy and Terms of Service. By closing this window the user confirms that they have read the information on cookie usage, and they accept the privacy policy and the way cookies are used by the portal. You can change the cookie settings in your browser.
Three membrane microdomains can be identified on docked vacuoles: ''outside'' membrane, not in contact with other vacuoles, ''boundary'' membrane that contacts adjacent vacuoles, and ''vertices,'' where boundary and outside membrane meet. In living cells and in vitro, vacuole fusion occurs at vertices rather than from a central pore expanding radially. Vertex fusion leaves boundary membrane within...
Vacuole fusion requires Secl 8p (NSF), Sec17p (α-SNAP), Ypt7p (GTP binding protein), Vam3p (t-SNARE), Nyv1p (v-SNARE), and LMA1 (low M, activity 1, a heterodimer of thioredoxin and I B2 ). LMA1 requires Sec18p for saturable, high-affinity binding to vacuoles, and Sec18p ''priming'' ATPase requires both Sec17p and LMA1. Either the sec18-1 mutation and deletion of I B2 , or...
Vacuoles (lysosomes) of the budding yeastSaccharomyces cerevisiaeare low copy-number organelles which are inherited from mother to daughter cells. We are interested in the molecular basis of the division and segregation of vacuole material and in the coordination of these events with the cell cycle. Using microscopic, genetic and biochemical methods, we have shown that vacuole inheritance is a regulated...
S. cerevisiae inherits its vacuole by projecting vacuole-derived membrane vesicles and tubules into the bud, where they fuse to establish the daughter vacuole. This homotypic fusion event can be assayed in vitro. It requires Sec17p and Sec18p, the homologs of the mammalian α-SNAP and NSF, which cooperate in multiple steps of membrane trafficking. We now report that Sec17p, Sec18p, and ATP are only...
The SecA subunit of E. coli preprotein translocase promotes protein secretion during cycles of membrane insertion and deinsertion at SecYEG. This process is regulated both by nucleotide binding and hydrolysis and by the SecD and SecF proteins. In the presence of associated preprotein, the energy of ATP binding at nucleotide-binding domain 1 (NBD1) drives membrane insertion of a 30 kDa domain of SecA,...
Set the date range to filter the displayed results. You can set a starting date, ending date or both. You can enter the dates manually or choose them from the calendar.