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The goal of this study was to analyse expression profiles of human epithelial host defence peptides in burned and unburned skin tissue, samples of which were obtained during debridements and snap-frozen in liquid nitrogen. Total RNA was isolated, and cDNA of epithelial host defence peptides and proteins (hCAP-18/LL-37, hBD1-hBD4, dermcidin, S100A7/psoriasin and RNAse7) was quantified by qRT-PCR. In...
In unstimulated cells, proteins of the nuclear factor κB (NF-κB) transcription factor family are sequestered in the cytoplasm through interactions with IκB inhibitor proteins. Tumor necrosis factor alpha (TNF-α) activates the degradation of IκB-α and the nuclear import of cytoplasmic NF-κB. Nuclear localization of numerous cellular proteins is mediated by the ability of the cytoskeleton, usually microtubules,...
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