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Although the N-terminally attached fatty acids are key structural elements of nonribosomally assembled lipopeptide antibiotics, little is known about the mechanism of lipid transfer during the initial step of biosynthesis. In this study, we investigated the activity of the dissected initiation module (C-A Glu -PCP) of surfactin synthetase SrfAA in vitro to gain further insights into the lipoinitiation...