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The native cell wall acid phosphatase (APase), WP-II, of tobacco XD-6 cells was purified by chromatographic procedures. The purified WP-II yielded one major band of M r of 60 kD on SDS-polyacrylamide gel electrophoresis(PAGE) after SDS treatment at 100°C for 5 min. The preparation, however, yielded an additional smear band with M r of 120 kD that retained phosphatase activity after...