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Summary We present a computational method that utilizes perturbation Monte Carlo and differential Monte Carlo methods, together with a nonlinear optimization algorithm, to solve inverse problems. Although motivated by biomedical applications, the solution method is quite general and can be applied to inverse problems in other fields. Each such problem involves the use of some form of radiation to...
We employ a clinical multiphoton microscope to monitor in vivo and noninvasively the changes in reduced nicotinamide adenine dinucleotide (NADH) fluorescence of human epidermal cells during arterial occlusion. We correlate these results with measurements of tissue oxy- and deoxyhemoglobin concentration during oxygen deprivation using spatial frequency domain imaging. During arterial occlusion, a decrease...
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