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The research on our flow-injection, label-free, non-faradaic impedimetric immunosensor for interferon-γ (IFN-γ) has been extended. The sensor is prepared by immobilization of anti-IFN-γ antibodies on a self-assembled monolayer (SAM) of acetylcysteine, deposited on polycrystalline gold. A multi-frequency impedance method is described, which allows time-resolved measurement of Nyquist plots. To these...