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Barley contains two forms (designated as low and high pI forms) of a potent inhibitor against limit dextrinase from barley malt. In this study the nature of the complex formed between the low pI inhibitor and limit dextrinase was analysed by electrospray ionization time-of-flight mass spectrometry. The complex was a 1:1 molar ratio of the enzyme and inhibitor and this ratio did not change in the presence...