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A new method is presented for the determination of 2′-deoxythymidine 5′-triphosphate and 2′-deoxycytidine 5′-triphosphate concentrations within human cells based on a DNA polymerase reaction directed by a palindromic oligonucleotide precursor. Two 19-mer oligonucleotide precursors are employed that contain a common 8-mer palindromic sequence followed by a sequence-specific insertion site and a 5′-oligodeoxythymidylate...
T-20 (pentafuside, DP-178), a 36-mer synthetic peptide derived from the HIV-1 gp41 transmembrane protein, is a selective and potent inhibitor of HIV-1 fusion (IC 50 =1 ng/ml) and infection in vitro. Pharmacokinetic studies in rodents revealed a surprisingly long half-life in circulation (t 1 =2.4 hr), with sustained levels above the IC 50 for at least 6 hr. Therefore,...
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