Search results for: William L. Stanford

Items from 1 to 20 out of 39 results

article

Rationally Designed 3D Hydrogels Model Invasive Lung Diseases Enabling High‐Content Drug Screening

Roger Y. Tam, Julien Yockell‐Lelièvre, Laura J. Smith, Lisa M. Julian, more

Cell behavior is highly dependent upon microenvironment. Thus, to identify drugs targeting metastatic cancer, screens need to be performed in tissue mimetic substrates that allow cell invasion and matrix remodeling. A novel biomimetic 3D hydrogel platform that enables quantitative analysis of cell invasion and viability at the individual cell level is developed using automated data acquisition methods...

article

Sol gel-derived hydroxyapatite films over porous calcium polyphosphate substrates for improved tissue engineering of osteochondral-like constructs

Whitaik David Lee, Rahul Gawri, Robert M. Pilliar, William L. Stanford, more

Acta Biomaterialia > 2017 > 62 > C > 352-361

Integration of in vitro-formed cartilage on a suitable substrate to form tissue-engineered implants for osteochondral defect repair is a considerable challenge. In healthy cartilage, a zone of calcified cartilage (ZCC) acts as an intermediary for mechanical force transfer from soft to hard tissue, as well as an effective interlocking structure to better resist interfacial shear forces. We have developed...

article

Direct reprogramming with SOX factors: masters of cell fate

Lisa M Julian, Angela CH McDonald, William L Stanford

Current Opinion in Genetics & Development > 2017 > 46 > C > 24-36

Over the last decade significant advances have been made toward reprogramming the fate of somatic cells, typically by overexpression of cell lineage-determinant transcription factors. As key regulators of cell fate, the SOX family of transcription factors has emerged as potent drivers of direct somatic cell reprogramming into multiple lineages, in some cases as the sole overexpressed factor. The vast...

article

Micro-RNA Profiling of Exosomes from Marrow-Derived Mesenchymal Stromal Cells in Patients with Acute Myeloid Leukemia: Implications in Leukemogenesis

Juliana Barrera-Ramirez, Jessie R. Lavoie, Harinad B. Maganti, William L. Stanford, more

Stem Cell Reviews and Reports > 2017 > 13 > 6 > 817-825

Gene regulatory networks in AML may be influenced by microRNAs (miRs) contained in exosomes derived from bone marrow mesenchymal stromal cells (MSCs). We sequenced miRs from exosomes isolated from marrow-derived MSCs from patients with AML (n = 3) and from healthy controls (n = 3; not age-matched). Known targets of mIRs that were significantly different in AML-derived MSC exosomes compared to controls...

article

Robust generation and expansion of skeletal muscle progenitors and myocytes from human pluripotent stem cells

Michael Shelton, Avetik Kocharyan, Jun Liu, Ilona S. Skerjanc, more

Methods > 2016 > 101 > C > 73-84

Human pluripotent stem cells provide a developmental model to study early embryonic and tissue development, tease apart human disease processes, perform drug screens to identify potential molecular effectors of in situ regeneration, and provide a source for cell and tissue based transplantation. Highly efficient differentiation protocols have been established for many cell types and tissues; however,...

article

Human pluripotent stem cells

Richard L. Carpenedo, Lisa M. Julian, William L. Stanford

Methods > 2016 > 101 > C > 1-3

article

Formula G1: Cell cycle in the driver's seat of stem cell fate determination

Lisa M. Julian, Richard L. Carpenedo, Janet L. Manias Rothberg, William L. Stanford

BioEssays > 38 > 4 > 325 - 332

Cell cycle dynamics has emerged as a key regulator of stem cell fate decisions. In particular, differentiation decisions are associated with the G1 phase, and recent evidence suggests that self‐renewal is actively regulated outside of G1. The mechanisms underlying these phenomena are largely unknown, but direct control of gene regulatory programs by the cell cycle machinery is heavily implicated...

chapter

Transcriptional Networks Regulating Embryonic Stem Cell Fate Decisions

Emily Walker, William L. Stanford

Stem Cell Biology and Regenerative Medicine > Regulatory Networks in Stem Cells > Molecular Regulation in Stem Cells > 87-100

A comprehensive understanding of the transcriptional regulation of embryonic stem cell (ESC) fate decisions will provide the key to their successful manipulation for therapeutic purposes as well as provide insight into the process of early embryogenesis. Traditional molecular and genetic approaches have been successful in identifying several essential regulators of pluripotency, notably Oct4, Nanog,...

article

Derivation and Expansion of PAX7-Positive Muscle Progenitors from Human and Mouse Embryonic Stem Cells

Michael Shelton, Jeff Metz, Jun Liu, Richard L. Carpenedo, more

Stem Cell Reports > 2014 > 3 > 6 > 1159

article

Sox17-Mediated XEN Cell Conversion Identifies Dynamic Networks Controlling Cell-Fate Decisions in Embryo-Derived Stem Cells

Angela C.H. McDonald, Steffen Biechele, Janet Rossant, William L. Stanford

Cell Reports > 2014 > 9 > 2 > 780-793

Little is known about the gene regulatory networks (GRNs) distinguishing extraembryonic endoderm (ExEn) stem (XEN) cells from those that maintain the extensively characterized embryonic stem cell (ESC). An intriguing network candidate is Sox17, an essential transcription factor for XEN derivation and self-renewal. Here, we show that forced Sox17 expression drives ESCs toward ExEn, generating XEN cells...

article

Derivation and Expansion of PAX7-Positive Muscle Progenitors from Human and Mouse Embryonic Stem Cells

Michael Shelton, Jeff Metz, Jun Liu, Richard L. Carpenedo, more

Stem Cell Reports > 2014 > 3 > 3 > 516-529

Cell therapies treating pathological muscle atrophy or damage requires an adequate quantity of muscle progenitor cells (MPCs) not currently attainable from adult donors. Here, we generate cultures of approximately 90% skeletal myogenic cells by treating human embryonic stem cells (ESCs) with the GSK3 inhibitor CHIR99021 followed by FGF2 and N2 supplements. Gene expression analysis identified progressive...

article

Inhibition of apoptosis in human induced pluripotent stem cells during expansion in a defined culture using angiopoietin-1 derived peptide QHREDGS

Lan T.H. Dang, Nicole T. Feric, Carol Laschinger, Wing Y. Chang, more

Biomaterials > 2014 > 35 > 27 > 7786-7799

Adhesion molecule signaling is critical to human pluripotent stem cell (hPSC) survival, self-renewal, and differentiation. Thus, hPSCs are grown as clumps of cells on feeder cell layers or poorly defined extracellular matrices such as Matrigel. We sought to define a small molecule that would initiate adhesion-based signaling to serve as a basis for a defined substrate for hPSC culture. Soluble angiopoeitin-1...

article

BRCA1 is a novel target to improve endothelial dysfunction and retard atherosclerosis

Krishna K. Singh, Praphulla C. Shukla, Adrian Quan, Mohammed Al-Omran, more

The Journal of Thoracic and Cardiovascular Surgery > 2013 > 146 > 4 > 949-960.e4

BRCA1, a tumor suppressor gene implicated in breast and ovarian cancers, exerts multiple effects on DNA repair and affords resistance against cellular stress responses. We hypothesized that BRCA1 limits endothelial cell apoptosis and dysfunction, and via this mechanism attenuates atherosclerosis.Loss and gain of function were achieved in cultured endothelial cells by silencing and overexpressing BRCA1,...

article

Feeder-independent derivation of induced-pluripotent stem cells from peripheral blood endothelial progenitor cells

Wing Y. Chang, Jessie R. Lavoie, Sarah Y. Kwon, Zhaoyi Chen, more

Stem Cell Research > 2013 > 10 > 2 > 195-202

Induced-pluripotent stem cells (iPSCs) are a potential alternative cell source in regenerative medicine, which includes the use of differentiated iPSCs for cell therapies to treat coronary artery and/or peripheral arterial diseases. Late-outgrowth endothelial progenitor cells (late-EPCs) are a unique primary cell present in peripheral blood that exhibit high proliferative capacity, are being used...

article

The mammalian gene function resource: the international knockout mouse consortium

Allan Bradley, Konstantinos Anastassiadis, Abdelkader Ayadi, James F. Battey, more

Mammalian Genome > 2012 > 23 > 9-10 > 580-586

In 2007, the International Knockout Mouse Consortium (IKMC) made the ambitious promise to generate mutations in virtually every protein-coding gene of the mouse genome in a concerted worldwide action. Now, 5 years later, the IKMC members have developed high-throughput gene trapping and, in particular, gene-targeting pipelines and generated more than 17,400 mutant murine embryonic stem (ES) cell clones...

article

Cytopenia induction by 5-fluorouracil identifies thrombopoietic mutants in sensitized ENU mutagenesis screens

Nicole M. Anderson, Zorana Berberovic, Elizabeth Berndl, Monica L. Bailey, more

Experimental Hematology > 2012 > 40 > 1 > 48-60

The ability of random mutagenesis techniques to annotate the mammalian genome can be hampered due to genetic redundancy and compensatory pathways that mask heterozygous mutations under homeostatic conditions. The objective of this study was to devise a pharmacologically sensitized screen using the chemotherapeutic drug, 5-fluorouracil (5FU), to induce cytopenia. 5FU dose was optimized in the 129/SvImJ,...

article

Forward and Reverse Genetics through Derivation of Haploid Mouse Embryonic Stem Cells

Ulrich Elling, Jasmin Taubenschmid, Gerald Wirnsberger, Ronan O'Malley, more

Cell Stem Cell > 2011 > 9 > 6 > 563-574

All somatic mammalian cells carry two copies of chromosomes (diploidy), whereas organisms with a single copy of their genome, such as yeast, provide a basis for recessive genetics. Here we report the generation of haploid mouse ESC lines from parthenogenetic embryos. These cells carry 20 chromosomes, express stem cell markers, and develop into all germ layers in vitro and in vivo. We also developed...

article

Integrating post‐transcriptional regulation into the embryonic stem cell gene regulatory network

Paul A. Cassar, William L. Stanford

Journal of Cellular Physiology > 227 > 2 > 439 - 449

Stem cell behavior is orchestrated as a multilayered, concert of gene regulatory mechanisms collectively referred to as the gene regulatory network (GRN). Via cooperative mechanisms, transcriptional, epigenetic, and post‐transcriptional regulators activate and repress gene expression to finely regulate stem cell self‐renewal and commitment. Due to their tractability, embryonic stem cells (ESCs) serve...

article

A novel ENU-generated truncation mutation lacking the spectrin-binding and C-terminal regulatory domains of Ank1 models severe hemolytic hereditary spherocytosis

Michael R. Hughes, Nicole Anderson, Steven Maltby, Justin Wong, more

Experimental Hematology > 2011 > 39 > 3 > 305-320.e2

Hereditary spherocytosis (HS) is a heterogeneous group of spontaneously arising and inherited red blood cell disorders ranging from very mild subclinical cases to severe and life-threatening cases, with symptoms linked directly to the severity of the mutation at the molecular level. We investigated a novel mouse model in which the heterozygotes present with the diagnostic hallmarks of mild HS and...

article

One-step generation of murine embryonic stem cell-derived mesoderm progenitors and chondrocytes in a serum-free monolayer differentiation system

Elaine Y.L. Waese, William L. Stanford

Stem Cell Research > 2011 > 6 > 1 > 34-49

Cartilage defects have limited capacity for repair and are often replaced by fibrocartilage with inferior mechanical properties. To overcome the limitations of artificial joint replacement, high-throughput screens (HTS) could be developed to identify molecules that stimulate differentiation and/or proliferation of articular cartilage for drug therapy or tissue engineering. Currently embryonic stem...

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INFONA - science communication portal

Search results for: William L. Stanford

Rationally Designed 3D Hydrogels Model Invasive Lung Diseases Enabling High‐Content Drug Screening

Sol gel-derived hydroxyapatite films over porous calcium polyphosphate substrates for improved tissue engineering of osteochondral-like constructs

Direct reprogramming with SOX factors: masters of cell fate

Micro-RNA Profiling of Exosomes from Marrow-Derived Mesenchymal Stromal Cells in Patients with Acute Myeloid Leukemia: Implications in Leukemogenesis

Robust generation and expansion of skeletal muscle progenitors and myocytes from human pluripotent stem cells

Human pluripotent stem cells

Formula G1: Cell cycle in the driver's seat of stem cell fate determination

Transcriptional Networks Regulating Embryonic Stem Cell Fate Decisions

Derivation and Expansion of PAX7-Positive Muscle Progenitors from Human and Mouse Embryonic Stem Cells

Sox17-Mediated XEN Cell Conversion Identifies Dynamic Networks Controlling Cell-Fate Decisions in Embryo-Derived Stem Cells

Derivation and Expansion of PAX7-Positive Muscle Progenitors from Human and Mouse Embryonic Stem Cells

Inhibition of apoptosis in human induced pluripotent stem cells during expansion in a defined culture using angiopoietin-1 derived peptide QHREDGS

BRCA1 is a novel target to improve endothelial dysfunction and retard atherosclerosis

Feeder-independent derivation of induced-pluripotent stem cells from peripheral blood endothelial progenitor cells

The mammalian gene function resource: the international knockout mouse consortium

Cytopenia induction by 5-fluorouracil identifies thrombopoietic mutants in sensitized ENU mutagenesis screens

Forward and Reverse Genetics through Derivation of Haploid Mouse Embryonic Stem Cells

Integrating post‐transcriptional regulation into the embryonic stem cell gene regulatory network

A novel ENU-generated truncation mutation lacking the spectrin-binding and C-terminal regulatory domains of Ank1 models severe hemolytic hereditary spherocytosis

One-step generation of murine embryonic stem cell-derived mesoderm progenitors and chondrocytes in a serum-free monolayer differentiation system

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