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The synergistic effect of temperature (25–65°C) and total specific energy input (0.55–1.11kWhkgDW−1) by pulsed electric field (PEF) on the release of intracellular components from the microalgae Chlorella vulgaris was studied. The combination of PEF with temperatures from 25 to 55°C resulted in a conductivity increase of 75% as a result of cell membrane permeabilization. In this range of temperatures,...