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Steady state fluorescence measurements, due to their relative simplicity and fast and easy implementation, are one of the most employed techniques for evaluating the non-covalent binding of small molecules to proteins. In the present review we discuss the main characteristics of solute binding and the experimental procedures that can be employed for evaluating both, the efficiency of the process and...
The effect of urea on the competition between Tl + and Na + ions to bind with the surface of dodecylsulfate micelles in aqueous solution has been studied by fluorescence techniques, employing methods based on fluorescence quenching data (FQD) and critical micelle concentration measurements (CMCM). The results obtained are interpreted in terms of selectivity coefficientsK(Tl + ...
We have investigated the effect of the incorporation of a linear (1-octanol) and a branched (2,6-dimethyl-4-heptanol) alkanol into large unilamellar vesicles of dipalmitoylphosphatidylcholine in the gel state upon several properties of the bilayer. The effect was evaluated by measurements of the rate of water outflow following a hypertonic osmotic shock, generalized polarization of 6-dodecanoyl-2-dimethyl...
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