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Experiments have been conducted into the unidirectional freezing of the aqueous solution of winter flounder antifreeze protein in a narrow gap between two cover glasses. A gap of 20μm, which is narrower than the gaps used by Coger (32μm) and Furukawa (60μm), has been adopted. It is confirmed that the local temperature near the ice/solution interface can be measured accurately with a near-infrared...