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Objective: Clinical application of vitrification for the cryopreservation of human blastocysts.Design: Clinical trial of vitrification of human blastocysts.Setting: Private assisted reproductive technology clinic.Patient(s): Supernumerary blastocysts after fresh blastocyst transfer were vitrified for subsequent transfer.Intervention(s): Culture of pronuclear embryos to the blastocyst stage in sequential...
Isolated single smooth muscle cells from the fundus of a guinea-pig stomach were permeabilized by use of streptolysin-O (0.5 U/ml). Most of the permeabilized cells responded to 0.6 μM Ca 2+ , but not to 0.2 μM Ca 2+ , with a resulting maximal cell shortening to approximately 71% of the resting cell length. These cells were relaxed again by washing with the Ca 2 ...
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