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A pure, active cytochrome b6f was isolated from the chloroplasts of the marine green alga, Bryopsiscorticulans. To investigate and characterize this cytochrome b6f complex, sodium dodecyl sulfate–polyacrylamide gel electrophoresis (SDS–PAGE), absorption spectra measurement and HPLC were employed. It was shown that this purified complex contained four large subunits with apparent molecular masses...
Cytochrome b 6 f complex with stoichiometrically bound β-carotene molecule was purified from spinach chloroplasts. The configuration of this β-carotene was studied by reversed-phase HPLC and resonance Raman spectroscopy. Both the absorption spectrum of this β-carotene in dissociated state and the Raman spectrum in native state can be unambiguously assigned to a 9-cis configuration. This finding...
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