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Perdeuterated pyridinium chloride–DMSO-d 6 is an effective solvent system for whole cell biomass dissolution and NMR characterization. Employing this solvent system, semi-quantitative 13 C– 1 H heteronuclear single quantum correlation (HSQC) spectroscopy of untreated, steam, dilute acid and lime pretreated poplar biomass samples was readily accomplished. In an effort to demonstrate...