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This paper proposes a non-chemical, mechanical giant liposome trapping method using a parylene filter with micrometer-order-sized holes. Giant liposomes can be useful reaction sites for biological assays, where immobilization of the liposomes is the key protocol for microscopic observation. The proposed mechanical trapping method using the parylene filter and gentle flow does not require pretreatments...
In this study, we purpose a centrifuge-based dynamic microarray system (CDM) for trapping micro-sized samples sequentially from only 10 μl of the samples. Our system enables us to make an array of microbeads only with a spin-coater. The air can be introduced to the microchannel to isolate and encapsulate the trapped beads by the specific speed rotation because the microbeads were fixed at the outlet...
This paper describes a dynamic microarray system for pairing adherent cells at single cell level. By introducing single cells cultured on mobile Parylene microplates into the system, we achieved pairing of single cells maintaining their physiological properties. By taking advantage of processibility of Parylene, we changed the shape and fabricated microgrooves on the plate surface. These modifications...
This paper describes a dynamic microarray device with pneumatic valves for trapping and releasing microbeads selectively. We fabricated thin membranes of polydimethylsiloxane (PDMS) inside our conventional dynamic microfluidic device. The membrane works as a pneumatically driven valve that changes the fluidic resistance, which ultimately determines the modes of the device: trapping, passing, or releasing...
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