Search results for: José Martı́nez-Costas
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Journal of Molecular Biology > 2004 > 341 > 2 > 361-374
We have recently shown that the avian reovirus non-structural protein μNS forms cytoplasmic inclusions in transfected cells and recruits σNS to these structures. In the present study we further demonstrate that μNS mediates the association of the major core protein λA, but not of σA or σC, with inclusions, indicating that the recruitment of viral proteins into avian reovirus factories has specificity...
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The M3 genome segment of avian reovirus 1733, which encodes the nonstructural protein μNS, is 1996 nucleotides long and contains a long open reading frame that is predicted to encode a polypeptide of 635 amino acid residues. Examination of the deduced amino acid sequence of μNS revealed the presence of two regions near its carboxyl terminus with a high probability of forming α-helical coiled coils...
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We have cloned and sequenced the L3 genome segment of avian reovirus strain 1733, which specifies the viral guanylyltransferase protein, λC. The L3 gene is 3907 nucleotides long and encodes, in a single large open-reading frame, a polypeptide of 1285 amino acid residues, with a calculated M r of 142.2 kDa. Expression of this gene in a baculovirus/insect cell system produced a recombinant protein...
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The avian reovirus S1 gene contains three partially overlapping, out-of-phase open reading frames (ORFs) that are highly conserved in all avian reovirus strains examined to date. The three S1 ORFs of the avian reovirus strain S1133 were individually expressed in bacterial cells, and their purified translation products used as antigens to raise specific polyclonal antibodies. With these antibodies...
