Search results for: Masaaki Hirose

Items from 1 to 20 out of 27 results

article

Chemical forms of radioactive Cs in soils originated from Fukushima Dai-ichi nuclear power plant accident studied by extraction experiments

Masaaki Hirose, Yoshikazu Kikawada, Atsushi Tsukamoto, Takao Oi, more

Journal of Radioanalytical and Nuclear Chemistry > 2015 > 303 > 2 > 1357-1359

We performed three-step sequential extraction experiments, with Milli-Q water, 1 M ammonium acetate solution and 0.11 M acetic acid as extractants of Cs, on soils radiologically contaminated by Fukushima Dai-ichi Nuclear Power Plant accident. Though aqueous solutions containing ammonium salts are effective to extract Cs from soils, the obtained overall extracted fractions of radioactive Cs by the...

article

Mobility of radioactive cesium in soil originated from the Fukushima Daiichi nuclear disaster: application of extraction experiments

Yoshikazu Kikawada, Masaaki Hirose, Atsushi Tsukamoto, Ko Nakamachi, more

Journal of Radioanalytical and Nuclear Chemistry > 2015 > 304 > 1 > 27-31

Extraction experiments on soil radioactively contaminated by the Fukushima Daiichi Nuclear Power Plant accident were conducted by using a variety of extractants to acquire knowledge on the mobility of radioactive cesium in soil. The experimental results revealed that cesium is tightly bound with soil particles and that radioactive cesium newly deposited on soil due to the accident had apparently a...

article

Carbon tribo-layer for super-low friction of amorphous carbon nitride coatings in inert gas environments

Pengfei Wang, Masaaki Hirose, Yoshinobu Suzuki, Koshi Adachi

Surface & Coatings Technology > 2013 > 221 > Complete > 163-172

Super-low friction of amorphous carbon nitride (CNx) coating in inert gas environment has generally been attributed to the formation of a uniform and homogeneous carbon-rich transfer film (denoted as tribo-film) on the mating ball surface in the ball-on-disk tribo-system. However, the role of tribo-film in terms of its appearance (visibility and amount), composition and structure in the low friction...

article

Design and synthesis of novel DFG-out RAF/vascular endothelial growth factor receptor 2 (VEGFR2) inhibitors: 3. Evaluation of 5-amino-linked thiazolo[5,4-d]pyrimidine and thiazolo[5,4-b]pyridine derivatives

Masaaki Hirose, Masanori Okaniwa, Tohru Miyazaki, Takashi Imada, more

Bioorganic & Medicinal Chemistry > 2012 > 20 > 18 > 5600-5615

Our aim was to discover RAF/vascular endothelial growth factor receptor 2 (VEGFR2) inhibitors that possess strong activity and sufficient oral absorption, and thus, we selected a 5-amino-linked thiazolo[5,4-d]pyrimidine derivative as the lead compound because of its potential kinase inhibitory activities and its desired solubility. The novel tertiary 1-cyano-1-methylethoxy substituent was designed...

article

Recombinant human serum albumin hydrogel as a novel drug delivery vehicle

Masaaki Hirose, Akira Tachibana, Toshizumi Tanabe

Materials Science & Engineering C > 2010 > 30 > 5 > 664-669

Serum albumin acts as a physiological carrier for various compounds including drugs. A hydrogel consisting of recombinant human serum albumin (rHSA) was prepared to take advantage of drug binding ability of albumin for a sustained drug release carrier. The hydrogel was prepared by mixing rHSA and dithiothreitol and casted to a polystyrene mold. Hydrogel formation was thought to occur through the intermolecular...

article

Recombinant human antithrombin expressed in Chinese hamster ovary cells shows in vivo efficacy on rat DIC model similarly to plasma-derived antithrombin regardless of different N-glycosylation

Masaaki Hirose, Minoru Tsukada, Fumihiro Hirayama, Yoshiji Kubo, more

Thrombosis Research > 2007 > 119 > 5 > 631-641

Plasma-derived human antithrombin (pAT) is used for the treatments of disseminated intravascular coagulation (DIC) and hereditary antithrombin deficiencies. We expressed recombinant human antithrombin (rAT) in Chinese hamster ovary (CHO) cells. The purified rAT is composed of 55% α-isoform and 45% β-isoform. The structure of the N-linked oligosaccharides of rAT is the same biantennary complex type...

article

Novel potent neuropeptide Y Y5 receptor antagonists: Synthesis and structure–activity relationships of phenylpiperazine derivatives

Toshiyuki Takahashi, Aya Sakuraba, Tomoko Hirohashi, Takunobu Shibata, more

Bioorganic & Medicinal Chemistry > 2006 > 14 > 22 > 7501-7511

A series of phenylpiperazine derivatives were synthesized and evaluated for their neuropeptide Y (NPY) Y5 receptor antagonistic activities. The benzindane portion of 2 was replaced by 1-phenylpiperazine, resulting in novel urea derivative 3f. Subsequent optimization of the phenylpiperazine template by substitution of the phenyl moiety resulted in a series of (2-methanesulfonamidephenyl)piperazine...

article

Overall carbohydrate-binding properties of Castanea crenata agglutinin (CCA)

Keiichi Nomura, Nobuyuki Takahashi, Masaaki Hirose, Sachiko Nakamura, more

Carbohydrate Research > 2005 > 340 > 12 > 2004-2009

The carbohydrate-binding properties of Castanea crenata agglutinin (CCA) were investigated by an enzyme-linked lectin absorbent assay. The binding ability of each carbohydrate was compared using IC ₅₀ values. CCA exhibited mannose/glucose specificity, as observed with many mannose-binding jacalin-related lectins. For oligosaccharides containing glucose, it has been shown that the degree of...

article

Purification and characterization of recombinant human antithrombin containing the prelatent form in Chinese hamster ovary cells

Shinobu Mochizuki, Kenmi Miyano, Masahide Kondo, Masaaki Hirose, more

Protein Expression and Purification > 2005 > 41 > 2 > 323-331

Antithrombin (AT) is a serine proteinase inhibitor and a major regulator of the blood coagulation cascade. AT in human plasma has two isoforms, a predominant α-isoform and a minor β-isoform; the latter lacks N-glycosylation at Asn 135 and has a higher heparin affinity. From the difference in its folding states, the AT molecule can be separated into three forms: a native form, a denatured and inactive...

article

Dynamic Mechanism for the Serpin Loop Insertion as Revealed by Quantitative Kinetics

Nobuyuki Takahashi, Kanako Terakado, Genichi Nakamura, Carolina Soekmadji, more

Journal of Molecular Biology > 2005 > 348 > 2 > 409-418

The serpin conformational change by insertion of the reactive center loop into β-sheet A plays a central role in multiple physiological consequences such as serine proteinase inhibition, latency and serpinopathic polymerization. To study the dynamic mechanism for the loop insertion, a novel kinetic method was established utilizing the ovalbumin mutant R339T/A352R; the loop insertion progressed after...

article

Iron-Binding Process in the Amino- and Carboxyl-Terminal Lobes of Ovotransferrin: Quantitative Studies Utilizing Single Fe ³⁺ -Binding Mutants

Ikuko Okamoto, Kimihiko Mizutani, Masaaki Hirose

Biochemistry > 2004 > 43 > 34 > 11118 - 11125

article

Cloning and characterization in Pichia pastoris of PNO1 gene required for phosphomannosylation of N-linked oligosaccharides

Masami Miura, Masaaki Hirose, Taeko Miwa, Shinobu Kuwae, more

Gene > 2004 > 324 > Complete > 129-137

The yeast Pichia pastoris PNO1 (Phosphomannosylation of N-linked Oligosaccharides) gene, which is involved in phosphomannosylation of N-linked oligosaccharides, was cloned using the Saccharomyces cerevisiae MNN4 gene [Glycobiology 6 (1996) 805] as a probe. The PNO1 open reading frame (ORF) encodes a type II membrane protein composed of 777 amino acid residues. Only in the short region extending from...

article

N-Acyl 6,7-dimethoxy-1,2,3,4-tetrahydroisoquinoline: The first orexin-2 receptor selective non-peptidic antagonist

Masaaki Hirose, Shin-ichiro Egashira, Yasuhiro Goto, Takashi Hashihayata, more

Bioorganic & Medicinal Chemistry Letters > 2003 > 13 > 24 > 4497-4499

The identification of potent and selective orexin-2 receptor (OX₂R) antagonists is described based on the modification of N-acyl 6,7-dimethoxy-1,2,3,4-tetrahydroisoquinoline analogue 1, recently discovered during high throughput screening (HTS). Substitution of an acyl group in 1 with tert-Leucine (tert-Leu), and introduction of a 4-pyridylmethyl substituent onto the amino function of tert-Leu improved...

article

Design and Synthesis of the Potent, Orally Available, Brain-Penetrable Arylpyrazole Class of Neuropeptide Y5 Receptor Antagonists

Nagaaki Sato, Toshiyuki Takahashi, Takunobu Shibata, Yuji Haga, more

Journal of Medicinal Chemistry > 2003 > 46 > 5 > 666 - 669

article

Loop-inserted and thermostabilized structure of P1-p1′ cleaved ovalbumin mutant R339T11Edited by R. Huber

Masayuki Yamasaki, Yasuhiro Arii, Bunzo Mikami, Masaaki Hirose

Journal of Molecular Biology > 2002 > 315 > 2 > 113-120

Ovalbumin is a member of a superfamily of serine proteinase inhibitors, known as the serpins. It is, however, non-inhibitory towards serine proteinases, and lacks the loop insertion mechanism common to the serpins due to unknown structural factors. Mutant ovalbumin, R339T, in which the P14 hinge residue is replaced, was produced and analyzed for its thermostability and three-dimensional structure...

article

Expression and Characterization of Recombinant Human Antithrombin III in Pichia pastoris

Shinobu Mochizuki, Nobuaki Hamato, Masaaki Hirose, Kenmi Miyano, more

Protein Expression and Purification > 2001 > 23 > 1 > 55-65

Antithrombin III (ATIII) is a member of the serpin superfamily and a major regulator of the blood coagulation cascade. To express recombinant human ATIII (rATIII) in the methylotrophic yeast Pichia pastoris, we constructed an rATIII expression plasmid which contained the ATIII cDNA encoding mature protein region connected with the truncated mAOX2 promoter and the SUC2 secretion signal, introduced...

article

Acid/Azole Complexes as Highly Effective Promoters in the Synthesis of DNA and RNA Oligomers via the Phosphoramidite Method

Yoshihiro Hayakawa, Rie Kawai, Akiyoshi Hirata, Jun-ichiro Sugimoto, more

Journal of the American Chemical Society > 2001 > 123 > 34 > 8165 - 8176

article

Domain closure mechanism in transferrins: new viewpoints about the hinge structure and motion as deduced from high resolution crystal structures of ovotransferrin N-lobe

Kimihiko Mizutani, Bunzo Mikami, Masaaki Hirose

Journal of Molecular Biology > 2001 > 309 > 4 > 937-947

The crystal structure of holo hen ovotransferrin N-lobe refined at 1.65 A resolution has been obtained. The final model gave an R-factor of 0.173 in the resolution range between 10.0 and 1.65 A. The comparison of the structure with previous high-resolution apo and Fe ³ ⁺ -loaded, domain-opened intermediate structures provides new viewpoints on the domain closure mechanism upon Fe ³...

article

Different binding sites for the neuropeptide Y Y1 antagonists 1229U91 and J-104870 on human Y1 receptors

Tetsuya Kannoa, Akio Kanatani, Sabina L.C. Keen, Sachie Arai-Otsuki, more

Peptides > 2001 > 22 > 3 > 405-413

The peptidic Y1 antagonist 1229U91 and the non-peptidic antagonist J-104870 have high binding affinities for the human Y1 receptor. These Y1 antagonists show anorexigenic effects on NPY-induced feeding in rats, although they have completely different structures and molecular sizes. To identify the binding sites of these ligands, we substituted amino acid residues of the human Y1 receptor with alanine...

article

Different binding sites for the Y1 antagonists 1229U91 and J-104870 on human Y1 receptors

Tetsuya Kanno, Akio Kanatani, Sabina L.C. Keen, Sachie Arai-Otsuki, more

Regulatory Peptides > 2000 > 94 > 1-3 > 15

Publication date

Set your own date range

INFONA - science communication portal

Search results for: Masaaki Hirose

Chemical forms of radioactive Cs in soils originated from Fukushima Dai-ichi nuclear power plant accident studied by extraction experiments

Mobility of radioactive cesium in soil originated from the Fukushima Daiichi nuclear disaster: application of extraction experiments

Carbon tribo-layer for super-low friction of amorphous carbon nitride coatings in inert gas environments

Design and synthesis of novel DFG-out RAF/vascular endothelial growth factor receptor 2 (VEGFR2) inhibitors: 3. Evaluation of 5-amino-linked thiazolo[5,4-d]pyrimidine and thiazolo[5,4-b]pyridine derivatives

Recombinant human serum albumin hydrogel as a novel drug delivery vehicle

Recombinant human antithrombin expressed in Chinese hamster ovary cells shows in vivo efficacy on rat DIC model similarly to plasma-derived antithrombin regardless of different N-glycosylation

Novel potent neuropeptide Y Y5 receptor antagonists: Synthesis and structure–activity relationships of phenylpiperazine derivatives

Overall carbohydrate-binding properties of Castanea crenata agglutinin (CCA)

Purification and characterization of recombinant human antithrombin containing the prelatent form in Chinese hamster ovary cells

Dynamic Mechanism for the Serpin Loop Insertion as Revealed by Quantitative Kinetics

Iron-Binding Process in the Amino- and Carboxyl-Terminal Lobes of Ovotransferrin: Quantitative Studies Utilizing Single Fe ³⁺ -Binding Mutants

Cloning and characterization in Pichia pastoris of PNO1 gene required for phosphomannosylation of N-linked oligosaccharides

N-Acyl 6,7-dimethoxy-1,2,3,4-tetrahydroisoquinoline: The first orexin-2 receptor selective non-peptidic antagonist

Design and Synthesis of the Potent, Orally Available, Brain-Penetrable Arylpyrazole Class of Neuropeptide Y5 Receptor Antagonists

Loop-inserted and thermostabilized structure of P1-p1′ cleaved ovalbumin mutant R339T11Edited by R. Huber

Expression and Characterization of Recombinant Human Antithrombin III in Pichia pastoris

Acid/Azole Complexes as Highly Effective Promoters in the Synthesis of DNA and RNA Oligomers via the Phosphoramidite Method

Domain closure mechanism in transferrins: new viewpoints about the hinge structure and motion as deduced from high resolution crystal structures of ovotransferrin N-lobe

Different binding sites for the neuropeptide Y Y1 antagonists 1229U91 and J-104870 on human Y1 receptors

Different binding sites for the Y1 antagonists 1229U91 and J-104870 on human Y1 receptors

Filter options

Publication date

Content availability

Publication type

Keywords

Data set

Journal

INFONA - science communication portal

Search results for: Masaaki Hirose

Add recipient

Sending message cancelled

Are you sure you want to cancel sending this message?

Send message

Filter options

Publication date

Date range setting

Set the date range to filter the displayed results. You can set a starting date, ending date or both. You can enter the dates manually or choose them from the calendar.

Content availability

Publication type

Keywords

Data set

Journal

Reporting an error / abuse

Sending the report failed

Accessibility options