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We have cloned a member of thefork head/HNF-3 family of transcription factors from the nematodeCaenorhabditis elegans.Within the predicted DNA binding domain, this gene, calledCe-fkh-1,is 75-78% identical to theDrosophila fork headand rat liver HNF-3 α, β, and γ genes.Ce-fkh-1mRNA is highly enriched in embryos. TheCe-fkh-1gene produces three major transcripts: the longest mRNA retains its original...
The Caenorhabditis elegans ges-1 gene (gut es terase No. 1 ) is expressed only in the intestinal lineage, beginning when the developing gut has only four to eight cells. We analyze the sequence requirements for this tissue-specific gene regulation by injecting deleted/mutated constructs of the ges-1 gene into a viable ges-1 (null) strain of worms and assaying heritably transformed embryos by esterase...