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The broad-spectrum antibiotic sulfamethoxazole (SMX) was chosen to assess its short- and long-term effects on denitrifying granules. The SMX concentration and pre-exposure time in batch testing influenced the denitrifying granule activity. In the continuous-flow experiments, no inhibitory effects on the upflow anaerobic sludge blanket performance were observed at SMX concentrations up to 100mgL−1,...