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We report here the successful 3D visualization of human placenta villous structures on the order of ∼1 mm3 by a combination of immunolabeling, rapid tissue clarification and laser scanning confocal microscopy. The resultant image sets exhibit a complex arrangement of villi and their contained vasculature that mirrors their arrangement in situ.
A self-cleaving hammerhead ribozyme targeted to codon 47 in β-amyloid precursor protein (βAPP) mRNA was cloned as a eucaryotic transcription cassette into the 3' UTR of enhanced green fluorescence protein (EGFP) mRNA, producing a C-terminal fusion mRNA. CMV promotor-driven vectors bearing this construct or a mutationally inactive ribozyme construct were transiently transfected into human embryonic...
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