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A rapid procedure for two‐dimensional pulsed field minigel electrophoresis (2D‐mini‐PFGE) with high throughput sample format was standardized to separate chromosomal DNA molecules of yeast. Molecules separation in the first dimension (1D) was done in 4.5 h using the contour‐clamped homogeneous electric field minichamber. 1D‐minigel was negatively stained with zinc‐imidazole and the lanes containing...
We standardized the zinc–imidazole negative staining method for detecting chromosomal-sized DNA molecules separated by pulsed field minigel electrophoresis. The best experimental conditions were as follows: separating large DNA molecules in minigels of 0.4cm thickness, further incubating them with 40mM ZnSO 4 solution, and finally incubating them with 0.1 and 2M imidazole solutions successively...
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