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An efficient procedure for the production of l-[4- 13 C]aspartic acid (4- 13 C-Asp) was investigated. In this procedure, phosphoenolpyruvate carboxylase originating from the methanol-assimilating microbe, Methylobacterium extorquens JCM 2805, was used for the production of labeled oxaloacetic acid from phosphoenol-pyruvate (PEP) and NaH 13 CO 3 ; the...