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Although most duplex DNAs are not immunogenic some synthetic DNAs such as polyl[d(Tm 5 C)] . poly-[d(GA)] are weakly immunogenic allowing the production of monoclonal antibodies. The specificity of one of these antibodies, Jel 172, was investigated in detail by a competitive solid-phase radioimmune assay. Jel 172 bound well to poly[d(TC)].poly[d(GA)] but not to other duplex DNAs such as poly[d(TTC)]...
Both brominated poly[d(GC)] and poly[d(Gm 5 C)] form stable left-handed Z-DNA structures at physiological ionic strengths. These two antigens were use to prepare monoclonal antibodies from immunized mice. The specificity of the antibodies was studied in detail with a solid-phase radioimmune assay as well as by means of competition experiments. Both immunogens produced several relatively non-specific...
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