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Cellular biology deals with studying the behavior of cells. Current time-lapse imaging microscopes help us capture the progress of experiments at intervals that allow for understanding of the dynamic and kinematic behavior of the cells. On the other hand, these devices generate such massive amounts of data (250GB of data per experiment) that manual sieving of data to identify interesting patterns...
As a tool to better understand the organization of the olfactory pathway three monoclonal antibodies have been isolated and characterized each having a unique staining pattern in the antenna and antennal lobe of Drosophila melanogaster. Monoclonal antibody F14-2D6 stains sensilla coeloconica and thick sensilla basiconica in the funiculus, Y1-3D10 stains only a few sensilla especially in and around...
Interleukin 6 (IL-6) is a cytokine involved in many aspects of the acute phase and immune responses. Cloning of rat IL-6 cDNA into the pET-21d expression plasmid under control of a bacteriophage T7 RNA polymerase promoter system allowed isopropylthio-galactopyranoside (IPTG)-inducible production of recombinant rat IL-6 inEscherichia coli. The cloning, expression and purification of rat IL-6 is described...
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