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Development of reliable and eco-friendly processes for synthesis of metallic nanoparticles is an important step in the field of application of nanotechnology. One of the options to achieve this objective is to use ‘natural factories’ such as biological systems. In this study, we report the synthesis of nanoparticles of silver by reduction of aqueous Ag+ ions with the culture supernatant of Bacillus...
Response surface methodology and central composite rotary design (CCRD) was employed to optimize a fermentation medium for the production of Nattokinase by Bacillus subtilis at pH 7.5. The four variables involved in this study were Glucose, Peptone, CaCl 2 , and MgSO 4 . The statistical analysis of the results showed that, in the range studied; only peptone had a significant effect...
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