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We were unable to confirm transient late gene expression using constructs of 18 genes that had been reported to supportAutographa californicamultinucleocapsid nucleopolyhedrovirus (AcMNPV) late gene expression when transfected intoSpodoptera frugiperdacells [Lu, A., and Miller, L. K. (1995).J. Virol.69, 975–982]. Three genes (orf66, orf68,andorf41) were included, all or in part, in the constructs...
The genome of theLymantria disparmultinucleocapsid nucleopolyhedrovirus (LdMNPV) was sequenced and analyzed. It is composed of 161,046 bases with a G + C content of 57.5% and contains 163 putative open reading frames (ORFs) of ≥150 nucleotides. Homologs were found to 95 of the 155 genes predicted for theAutographa californicaMNPV (AcMNPV) genome. More than 9% of the LdMNPV genome was occupied by 16...