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As one of the best candidates for simulating the microgravity conditions in space, low gravity environments provided by applying an upward magnetic force have been considered to grow protein crystals. Since 2002, the stable and long-time durable microgravity generated by a superconducting magnet has been available for protein crystal growth. In this paper, for the first time, we grew protein crystals...
Abstract The seeds of a Japanese terrestrial orchid (Bletilla striata Rchb.f.) were germinated and cultured on solidified new Dogashima (ND) medium for 10 days. These embryos were then precultured on ND medium supplemented with 0.3 m sucrose for 3 days at 25C in continuous dark. The embryos were then overlaid with a mixture of 2 m glycerol and 0.4 m sucrose for 15 min at 25C and finally dehydrated...
Purpose and Methods: Cyclodextrin glucano-transferase (CGTase) catalyzes the conversion of starch and related [alpha ]-1,4-glucans to cyclodextrins (CDs) through an intramolecular transglycosylation. Native CGTase from alkalophilic bacterium mainly produces a mixture of [alpha ]-, [beta ]-, y-CDs. However, the mutation of His233 to Asn (H233N-CGTase) does not produce [alpha ]-CD. To understand the...
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