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The rumen anaerobic fungi Orpinomyces joyonii A4 and Caecomyces communis JB1 were grown on microcrystalline cellulose (MC) and alfalfa hay. The cellular distribution of cellulases produced by these organisms was monitored. Fungal cultures were separated into extracellular, intracellular and cell wall fractions and assayed for endoglucanase (EG) and β-glucosidase activity. In both fungal isolates,...
A new microplate phospholipase D (PLD) assay has been developed on the basis of a two-step technique with colorimetric determination of the reaction product choline. The described method was optimized concerning its linearity, reproducibility and concentrations of reaction components. The results obtained with the microplate technique in the analysis of different PLD samples were compared with those...
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