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To determine if ethanol vapor is reinforcing, chambers with two nose-poke devices were used. First, C57BL/6J mice were trained to nose-poke at one (FR port) and then to hold a nose-poke for at least 1s at the second (reward port) for 10% sucrose reinforcement. When mice held reward port nose-pokes for ≥1s on 95% of session trials, ethanol vapor (0.05%, w/v) or clean air replaced sucrose. In experiment...
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