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Okadaic acid (OA), a key diarrheic shellfish poisoning (DSP) toxin will possibly arouses DSP symptoms by consuming the contaminated shellfish. The DSP toxins are stable at high temperatures, and long-term DSP toxicity is carcinogenic. Therefore a fast and reliable analytical method for the detection of OA and analogues in shellfish is worth developing. In this paper, a direct competitive ELISA (dcELISA)...
The recombinant single-chain trispecific antibody against three toxins including Okadaic acid (OA), saxitoxin (STX) and tetrodotoxin (TTX) was developed. Three single-chain variable fragment (ScFv) genes were linked via linkers (Gly4-Ser)3 to construct fusion ScFv gene OST-ScFv through splicing by overlapping extension. Fusion gene OST-ScFv was 2 256 bp, encoding 752 amino acids, and was inserted...
The objectives of this study were to evaluate the effects of different maturity on A.sparsifolia silage quality. Bud appearing (LF) , first flower appearing(LC), full blooming(LS), and first fruit appearing(LJ) A. sparsifolia were used in this experiment. Fore forages were harvested, chopped to ensiling in wide-mouth glass bottle, 150 days after ensiling, silages were sensorially evaluated and sampled...
Histone acetyltransferases (HATs) and histone deacetylases (HDACs) conduct many critical functions through nonhistone substrates in metazoans, but only chromatin-associated nonhistone substrates are known in Saccharomyces cerevisiae. Using yeast proteome microarrays, we identified and validated many nonchromatin substrates of the essential nucleosome acetyltransferase of H4 (NuA4) complex. Among these,...
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